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DC Field | Value | Language |
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dc.contributor.author | Ayala Guanga, Luis Eduardo | - |
dc.contributor.author | Samaniego Campoverde, Jorge Xavier | - |
dc.contributor.author | Nieto Escandon, Pedro Emilio | - |
dc.contributor.author | Rodas Carpio, Ermes Ramiro | - |
dc.contributor.author | Dutan Sanango, Jorge Bolivar | - |
dc.contributor.author | Calle Ortiz, Guido Rigoberto | - |
dc.contributor.author | Murillo Apolo, Yury Agapito | - |
dc.contributor.author | Vazquez Mosquera, Juan Mesias | - |
dc.contributor.author | Argudo Garzon, Daniel Ernesto | - |
dc.contributor.author | Perea Ganchou, Fernando Pedro | - |
dc.date.accessioned | 2018-06-16T14:41:00Z | - |
dc.date.available | 2018-06-16T14:41:00Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1682-3419 | - |
dc.identifier.uri | http://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13816 | - |
dc.description | The objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control).The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; however, about 50% of the type B, C and D COCs of T1 and T2 were BCB+. It is concluded that the selection of COC based on morphological characteristics is a reliable method only for type A and has a 50% error for COC type B, C and D and, therefore, the application of the BCB test allows to improve this selection non-invasively | - |
dc.description.abstract | The objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control).The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; however, about 50% of the type B, C and D COCs of T1 and T2 were BCB+. It is concluded that the selection of COC based on morphological characteristics is a reliable method only for type A and has a 50% error for COC type B, C and D and, therefore, the application of the BCB test allows to improve this selection non-invasively | - |
dc.language.iso | es_ES | - |
dc.source | Revista de Investigaciones Veterinarias del Perú | - |
dc.subject | Bovine Coc | - |
dc.subject | Oocyte Morphology | - |
dc.subject | Bcb | - |
dc.subject | G6pdh | - |
dc.title | Competencia del ovocito bovino obtenido por ovum pick-up valorado mediante el azul brillante de cresilo | - |
dc.title.alternative | Competence of the bovine oocyte obtained by ovum pick-up as evaluated by the bright cresyl blue test | - |
dc.type | ARTÍCULO | - |
dc.ucuenca.idautor | 0102635463 | - |
dc.ucuenca.idautor | 0105163489 | - |
dc.ucuenca.idautor | 0102840576 | - |
dc.ucuenca.idautor | 0102979424 | - |
dc.ucuenca.idautor | 0301093647 | - |
dc.ucuenca.idautor | 0300977238 | - |
dc.ucuenca.idautor | 0702650136 | - |
dc.ucuenca.idautor | 0103055554 | - |
dc.ucuenca.idautor | 0104461165 | - |
dc.ucuenca.idautor | 0151638103 | - |
dc.identifier.doi | 10.15381/rivep.v29i2.13816 | - |
dc.ucuenca.version | Versión publicada | - |
dc.ucuenca.areaconocimientounescoamplio | 08 - Agricultura, Silvicultura, Pesca y Veterinaria | - |
dc.ucuenca.afiliacion | Ayala, L., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Samaniego, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Nieto, P., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Rodas, E., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Dutan, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Calle, G., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Murillo, Y., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Vazquez, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Argudo, D., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Perea, F., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.correspondencia | Ayala Guanga, Luis Eduardo, luis.ayala@uceunca.edu.ec | - |
dc.ucuenca.volumen | volumen 29, número 2 | - |
dc.ucuenca.indicebibliografico | SCOPUS | - |
dc.ucuenca.factorimpacto | H4 | - |
dc.ucuenca.cuartil | Q3 | - |
dc.ucuenca.numerocitaciones | 0 | - |
dc.ucuenca.areaconocimientofrascatiamplio | 4. Ciencias Agrícolas | - |
dc.ucuenca.areaconocimientofrascatiespecifico | 4.4 Biotecnología Agricola | - |
dc.ucuenca.areaconocimientofrascatidetallado | 4.4.2 La BioTecnología y los Alimentos | - |
dc.ucuenca.areaconocimientounescoespecifico | 084 - Veterinaria | - |
dc.ucuenca.areaconocimientounescodetallado | 0841 - Veterinaria | - |
dc.ucuenca.urifuente | http://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria | - |
Appears in Collections: | Artículos |
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