Comparación de dos protocolos anestésicos utilizados para ovariohisterectomía en perras y su impacto en la función renal mediante análisis sanguíneos y urianálisis

Abstract

The objective of this research was to compare two anesthetic protocols used for ovariohysterectomy in bitches and their impact on renal function by blood analysis and urinalysis. Forty females were used, apparently healthy, without distinction of breed and distributed randomly to receive two anesthetic protocols during surgery (n = 20 patients per protocol). At 72 hours, post-surgery blood samples were obtained to evaluate protein, urea and creatinine; also, a urine sample was collected by ultrasound-guided cystocentesis for measuring specific gravity thereof. A completely randomized design (DCA) was used with the two anesthetic protocols (conventional and alternative) considered as treatments (T1 and T2) and twenty repetitions of each. The data obtained were systematized in Excel and analyzed with the statistical software SPSS version 23 ®. Outcome variables were urea, creatinine, serum protein and urine density, which were analyzed for normality (Shapiro Wilk test, P> 0.05) and homogeneity of variance (Levene test, P>0, 05). Finally, one factor analysis of variance (ANOVA) as parametric analysis, and the "Mann Whitney U" test as non-parametric analysis we applied, depending on the normality and homogeneity of variances. All tests were analyzed with 5% significance. It was established that the variable at time 0 (before surgery) most of them fulfilled the assumptions of normality and homogeneity of variance (test Shapiro Wilk, P> 0.05, Levene test, P> 0.05). When comparing protocols with respect to renal function parameters, there was no significant difference between them (P> 0.05). At 72 hours after surgery, a greater variability of the results in almost all variables (Levene test, P<0.05) was evidenced by parametric and non-parametric tests, which resulted in an increase in the standard error (EE) of serum protein and urine density. In conclusion, it was evidenced that there were no significant differences (P> 0.05) in the averages of each variable under study between the two anesthetic protocols.