Eficacia de la radiación ultravioleta en la esterilización del plasma seminal porcino y su comportamiento sobre calidad espermática

Abstract

The objective of the present work was to evaluate the effect of Ultraviolet Short Wave Radiation (UV-C) as a Porcine Seminal Plasma sterilizer and its behavior on sperm quality. Where a random Blocks design was used, consisting of 4 boars from which 4 ejaculated each were divided into 5 aliquots for the raised treatments giving a total of 80 experimental units. The treatments were: A.- no exposure; B.- 5 minutes of exposure; C.- 10 minutes of exposure; D.- 15 minutes of exposure, and E.- 20 minutes of exposure. A 254nm, 12W UV-C radiation sterilization chamber was used. Concentration of CFU / ml, progressive individual motility, vitality, abnormalities and sperm membrane integrity were evaluated. Significant bacterial reduction was obtained from 15 minutes of exposure to ultraviolet radiation type C obtaining 93.8% of sterilized samples. The parameters of sperm vitality, abnormalities and membrane integrity did not show significant differences between treatments (P> 0.05), whereas progressive sperm motility was different (p <0.05), being lower in semen reconstituted with semen plasma With greater exposure to UV-C radiation. It is concluded that UV-C radiation reduces bacterial load significantly as the exposure time to UV-C increases.