Diagnóstico de enfermedades de transmisión sexual mediante PCR tiempo real en mujeres indígenas del Ecuador 2016

Abstract

Background Screening test for the detection of sexually transmitted diseases, some of them producing cancer such as human papilloma virus, besides being factors of infertility and infertility, depends increasingly on molecular techniques. An important challenge for developing countries like Ecuador has been the lack of availability and costs of tests based on the detection of the DNA of the microorganism. In this study, microorganisms of sexual transmission were diagnosed by qPCR in women of indigenous nationality Ecuador.

Methodology Inside the laboratory of molecular biology of the Cuenca University, a descriptive study was carried out to diagnose sexually transmitted microorganisms in cervical samples using molecular techniques of HPV-28 and STI-7 Anyplex qPCRs. The samples came from woman indigenous from the provinces of Loja, Morona Santiago and Cañar.

Results 396 samples were analyzed, 120 samples were extracted in Saraguro, 131 in Quilloac community, 145 in Seville Don Bosco community. 135 samples showed high risk and low oncogenic HPV risk genotypes, also 362 samples with other sexually transmitted microorganisms.

Conclusions Implementing molecular techniques such as qPCR, for the diagnosis of microorganisms of sexual transmission are fast, simple with a high sensitivity and specificity