Cuantificación de glucosa, fructosa y sacarosa en muestras de pulpa y jugo de uvilla (Physallis Peruviana), tomate de árbol (Solanum Betaceum) y manzana (Pyrus Malus) por espectroscopia infrarroja por transformadas de Fourier (FTIR)

Abstract

The present work covers the quantification of glucose, sucrose and fructose in pulp and juice samples for three Andean fruits: tree tomato or tamarillo (Solanum betaceum), uvilla (Physalis peruviana L.) and apple (Pyrus malus) using Fourier-transform infrared spectroscopy (FTIR) in the middle infrared zone (700-4000 〖cm〗^(-1)). The quantification of sugars was performed by means of calibration curves, preparing serial dilutions of 20% w/v of glucose, fructose and sucrose of purity ≥ 99.5%. Working solutions from 0.5 to 5% w/v were used to acquire infra-red spectra in absorbance mode. Once the linear calibration model was obtained, the sugars were quantified on the samples and the data were compared with the refractometry method. Data comparison allowed us to validate the results of sugar concentration on the juice of the fruits included in this study. However, data for pulp samples were not suitable for validations due to the lack of information on the applied methods for reading these samples by FTIR spectroscopy.