Estandarización de un bioensayo de actividad antiparasitaria utilizando como organismo modelo a Fasciola hepática

Abstract

This paper aims to standardize a bioassay to assess potential antiparasitic naturally occurring substrates, compared its biocide effect with the action of a drug of reference.

The experimental work aimed used Fasciola hepatica as a model organism. Adult forms of the parasite were gathered from infected livers from bovine animals slaughtered in the Town hall Slaughterhouse, EMURPLAG. Trematodes were isolated by means of post-morten observation of fasciolasis, and identification of pathological lesions caused by the parasite. Adult flukes were collected from longitudinal slits performer in infested livers.

Before performing the bioassay, we monitored the viability of trematodes for 24 hours. The antiparasitic activity of plants extracts was tested in a seriate dilution assay. Three solutions of plants extracts at different concentrations were seeded to the mantainance media to test the parasites survival under these conditions. After a 24-hour period, we evaluated the trematodes survival, and the effectivity of the extracts was expressed as effectiveness percentage, related to the number of dead flukes. The results of this experimental work produced a methodology to asess antiparasitic activity "in vitro" at standard working conditions of a research laboratory. This technique will evaluate the potential of naturally occurring substances as anthelmintics and identify promising plant substrates as antiparasitic, forming the basis for further studies focused on developing new antiparasitic bioproducts.