Evaluación del crecimiento in vitro de plantulas de orquídea dacyglossumedwardii en medios de cultivo simples con suplementos orgánicos frente a medio de cultivo murashige y skoog modificado como testigo

Abstract

Orchids are plant species threatened due to an irrational exploitation for its ornamental value. Micro propagation methods must be optimized to improve massive reproduction of these species. The aim of the present study was to assess the “in vitro” growth of orchids from the specie Dacyglossumedwardii in simple culture media enriched with organic supplements: banana, green banana flour and potato puree. Murashige&Skoog modified medium was used as a control, to demonstrate the feasibility to replace simple media for the conventional medium. Green banana flour enriched with sacarose presented better results increasing plant growth up to 3,3 cm and survival percentage to 98.74%. Potato puree and conventional medium yielded plant growth of 3 and 2,8 cm and survival percentage of 97,5 and 91,25%, respectively. Media were formulated with an initial pH of 5.6. This value, considered optimum, experiment variations during the plant growth. Media with acidic pH, compared with 5.6 were more effective compared with basic pH media.